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. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: J Neurosci. 2011 Jan 5;31(1):247–261. doi: 10.1523/JNEUROSCI.4589-10.2011

Fig. 3. PRX2 protects the nigrostriatal dopaminergic system from 6-OHDA-induced degeneration in mice.

Fig. 3

(A) Brain sections from the striatum and SNc showing TH immunoreactivity 3 weeks after striatal infusion of saline or 6-OHDA (3.0 µg) preceded by brain inoculation of either empty lentiviral vector (Lenti) or Lenti-PRX2. Arrows indicate the SNc region lesioned by 6-OHDA. (B) Optical measurement of striatal TH immunoreactivity 3 weeks after the injections indicated. Controls were injected with saline. (C) The numbers of TH-positive neurons in the ipsilateral SNc (injected side) were counted using non-biased stereological methods 3 weeks after the injections indicated. (D) High-performance liquid chromatography-electrochemical detection data of catecholamine levels from the ipsilateral striatum 3 weeks following the indicated injections. Catecholamines are expressed as picomoles/mg of fresh, wet tissue. (E) Apomorphine-induced circling before 6-OHDA injection (Pre) or 2 and 3 weeks after the injections indicated is shown as the number of turns in the first 30 min after intraperitoneal injection of apomorphine. (F) Spontaneous turning using the corner test before 6-OHDA injection (Pre) or 3 weeks after the injections indicated. The mean of left/right turns ratio out of 10 trials/session are reported. *p<0.05; **p<0.01; ***p<0.001 vs. contralateral or saline control; #p<0.05; ##p<0.01 vs. 6-OHDA plus empty vector (Lenti). n=10–12 per group for all experiments.