FIG. 6.

By concatenating multiple optical files, it is possible to track the activity of individual neurons over multiple imaging sessions. The top traces show 10 of the 45 neuronal independent components returned by ICA performed on a file concatenated from 5 separate optical files acquired from the dorsal pedal ganglion of Tritonia over 24 min. The different optical recording files are shown separated by spaces. The bottom traces show intracellular recordings from 2 impaled pedal ganglion neurons that were acquired simultaneously with the optical data. The red intracellular recording trace corresponds exactly, spike-for-spike, to the red component and the green intracellular recording trace corresponds exactly, spike for-spike, to the green component. In optical files 1, 2, and 4, current was injected into the impaled neurons to make them fire action potentials and in optical files 3 and 5, a swim motor program was elicited by pedal nerve 3 stimulation (10 V, 10 Hz, 2 s, 5 ms pulses, stimuli indicated by arrows).