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. Author manuscript; available in PMC: 2011 Aug 9.
Published in final edited form as: J Neurosci. 2011 Feb 9;31(6):2035–2051. doi: 10.1523/JNEUROSCI.5634-10.2011

Figure 6.

Figure 6

Effect of NFκB inhibition on the PKCδ immunoreactivity in the primary dopaminergic neurons. A, Primary midbrain cultures were treated with or without 100 µg/ml of SN-50 for 24 h. Cultures were immunostained for TH (green) and PKCδ (red). The nuclei were counterstained by Hoechst 33342 (blue). Images were obtained using a Nikon TE2000 fluorescence microscope (magnification 60×). Scale bar, 10µm. Representative immunofluorescence images are shown. The insert shows a higher magnification of the cell body area. B, Immunofluorescence quantification of PKCδ in TH-positive neurons. Fluorescence immunoreactivity of PKCδ was measured from TH-neurons in each group using Metamorph software. Values expressed as percent of control group are mean ± SEM and representative for results obtained from three separate experiments in triplicate (**p<0.01).