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. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: J Cell Mol Med. 2012 Jan;16(1):160–173. doi: 10.1111/j.1582-4934.2011.01282.x

Panel A: miRNA was isolated and profiling by hybridization to miRNA-specific probes on epoxy-coated slides. Cluster analysis identifies a group of miRNA that are increased in expression in HCC cancer stem cells (HSC-SR and HSC-DF) and reduced in hepG2 cells when compared to normal human liver stem cells (Cluster 2). An enlarged view of this group, containing 11 miRNAs including members of let-7 and miR-181 family is shown. Lower middle panel illustrated representative Northern blot analysis of let-7a and miR-181a using total RNA isolated from four cell lines. This cluster of miRNAs are among the group, which increased in HSC-SR by 4-fold, and p<0.05 when compared to HepG2 cells (lower right panel). Panel B: The expressions of mature let-7a, let-7b and miR-181a miRNAs were validated using real-time PCR and correlated with the pattern in the miRNA array. Data represent mean ± standard error from four separate experiments.

Panel A: miRNA was isolated and profiling by hybridization to miRNA-specific probes on epoxy-coated slides. Cluster analysis identifies a group of miRNA that are increased in expression in HCC cancer stem cells (HSC-SR and HSC-DF) and reduced in hepG2 cells when compared to normal human liver stem cells (Cluster 2). An enlarged view of this group, containing 11 miRNAs including members of let-7 and miR-181 family is shown. Lower middle panel illustrated representative Northern blot analysis of let-7a and miR-181a using total RNA isolated from four cell lines. This cluster of miRNAs are among the group, which increased in HSC-SR by 4-fold, and p<0.05 when compared to HepG2 cells (lower right panel). Panel B: The expressions of mature let-7a, let-7b and miR-181a miRNAs were validated using real-time PCR and correlated with the pattern in the miRNA array. Data represent mean ± standard error from four separate experiments.