Fig. 1.
Effect of footshock on P-CREB in the NAS. (A) Western immunoblot of P-CREB in the NAS after exposure to footshock. Control rats were treated identically in all respects except they did not receive footshock. Exposure to footshock significantly elevated P-CREB in the NAS. GAPDH was used to normalize protein loading in each lane; data are expressed as the mean (±SEM) P-CREB/GAPDH ratio. *P<0.05, Student’s t-test. Effects were localized to the NAS by first dissecting the NAC (B) and then obtaining the NAS in a second, crescent-shaped punch (C). Number (mm) depicts distance from bregma; scale bar=500 µm