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. Author manuscript; available in PMC: 2013 Feb 6.
Published in final edited form as: J Neurosci. 2011 Mar 2;31(9):3470–3483. doi: 10.1523/JNEUROSCI.2768-10.2011

Figure 4. REST loss of function increases neuronal protein expression during astrocytic differentiation.

Figure 4

Purified OPCs were infected with retroviruses expressing either DnREST and/or GFP alone and grown for 5 days in 2A differentiation media. A. The percent of the infected cells that were positively stained with antibodies against GFAP and TUJ1. Error bars represent the standard deviation, n=3, ** P value < 0.001 B. Immunoblot analysis shows the expression of neuronal proteins in the DnREST infected cells after 5 days in 2A differentiation media. C. Immunofluorescence staining showing the expression of TUJ1 (red) in the infected cells (green) bar=50µm.