Figure 8. REST over expression induces 04 expression.
A. OPCs were nucleofected with either pMAXeGFP or a mixture of pMAXeGFP and full-length REST, grown for 3 days at clonal densities in defined media and the percentage of clones containing 04-postive cells determined (n=3, P<0.005). B. 3-dimensional scatter plots of a typical experiment. The number of 04-negative clones decreased and there was a general shift to clones containing higher percentages of 04-positive cells after REST over-expression (REST OE).