Table 1. Gene changes during differentiation.

OPCs were infected with control or DnREST expressing retroviruses and grown in proliferation media for 5 days. Cells were switched to either oligodendrocyte media or 2A differentiation media for 3–4 days. Total RNA was collected and transcript levels were analyzed by real-time RT-PCR. Relative fold difference in gene expression in DnREST infected cells is shown relative to control infected cells. The standard deviation (SD) was taken from 4–6 total PCR runs from 2 separate experiments.

Oligodendrocyte			2A
Gene Name	Relative Fold Difference	Gene Name	Relative Fold Difference
CelsR3	10.48 ± 4.16 **	SCG10	13.73 ± 2.24 **
SCG10	6.85 ± 0.25 **	CelsR3	11.33 ± 3.92 **
Snap25	5.25 ± 0.35 **	Snap25	4.88 ± 1.09 **
NFM	4.89 ± 0.98 **	Nestin	3.65 ± 2.05 *
Myt1	3.61 ± 0.12 **	Myt1L	2.95 ± 0.78 **
βIIITub	2.08 ± 0.28 **	βIIITub	2.93 ± 0.27 **
Syn1	1.96 ± 0.24 **	ATOH	2.50 ± 0.08 **
Nestin	1.83 ± 0.03 **	DCX	2.42 ± 1.17
Hes5	1.82 ± 0.05 **	NGN3	2.36 ± 0.07 **
Hes1	1.76 ± 0.75	NeuroD1	2.29 ± 1.13
Syt2	1.75 ± 0.95	NF-M	2.03 ± 0.02 **
Calb1	1.71 ± 0.08 **	Myt1	1.98 ± 0.15 **
Myt1L	1.58 ± 0.07 **	Hes1	1.71 ± 0.13 **
Olig2	1.49 ± 0.74	Syn1	1.55 ± 0.29 *
ASCL1	1.40 ± 0.04 **	Nkx2.2	1.48 ± 0.20 **
DCX	1.35 ± 0.06 **	NGN2	1.48 ± 0.02 **
PLP	NC	Calb1	1.47 ± 0.09 **
Sox2	NC	GluR2	1.40 ± 0.07 **
PDGFaR	NC	Olig1	1.39 ± 0.03 **
GluR2	NC	Olig2	1.25 ± 0.11 *
NG2	NC	B-actin	NC
Olig1	NC	Sox2	NC
Sox10	NC	ID4	NC
YY1	NC	YY1	NC
GFAP	NC	ASCL1	NC
CTNNb1	NC	Hes5	NC
Id2	NC	PLP	NC
Nkx2.2	NC	PDGFaR	NC
B-actin	NC	ID2	NC
Id4	NC	CNP	0.74 ± 0.22
MOBP	0.79 ± 0.07 **	NG2	0.68 ± 0.36
MBP	0.74 ± 0.05 **	MOBP	0.66 ± 0.38
MAG	0.72 ± 0.03 **	MBP	0.65 ± 0.05 **
CNP	0.50 ± 0.31 *	MAG	0.58 ± 0.18 **
		GFAP	0.41 ± 0.11 **
		Brca1	0.39 ± 0.02 **

NC= no change,

^*P value < 0.05,

^**P value < 0.005.

Where not indicated, the change was not statistically significant.