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. Author manuscript; available in PMC: 2011 Mar 15.
Published in final edited form as: Opt Express. 2010 Dec 20;18(26):27499–27510. doi: 10.1364/OE.18.027499

Fig. 1.

Fig. 1

A schematic of the experimental set-up of Holographic Opto-fluidic Microscopy (HOM). The sample to be imaged flows within a micro-fluidic channel, due to either electro-kinetic or pressure driven motion, and is illuminated by partially coherent light emanating from a large aperture (~0.05-0.1mm) placed ~50-100 mm from the sample. The micro-channel is placed directly on a digital sensor array with no additional manipulation of the sensor-chip. (Not to scale.) The propagation distance between the aperture and the sample allows for spatial coherence to develop at the sample plane, and light scattered by the sample interferes with the background light to form a hologram on the sensor array plane.