Table 2. Summary Data from Single Rod Measurements.
First five columns summarize properties of responses to dim flashes - peak: peak amplitude of the estimated single photon responses using a fixed collecting area of 0.5μm2; tp: time-to-peak of single photon response; ti: integration time or area under the normalized single photon response; τr: exponential constant describing single photon response recovery phase. Dark current, rmax, is calculated from saturated responses. i0 is the strength of the flash producing a half-maximal response, and tp(i0) is the time-to-peak of that response. τd is the dominant time constant for decay of light-activated PDE activity (see Fig. 5).
| Protocol | peak (pA) | tp (ms) | ti (ms) | τr (ms) | rmax (pA) | i0 (photons μm−2) | tp (i0) (ms) | τD (ms) |
|---|---|---|---|---|---|---|---|---|
| L-15/Locke’s | 0.24 ± 0.03 (12) | 127 ± 5 | 240 ± 11 | 174 ± 8 | 11.3 ± 0.5 (30) | 57.4 ± 2.3 (15) | 103 ± 4 | 210 ± 16 (21) |
| Warm L-15/Locke’s | 0.56 ± 0.08 (15) | 159 ± 8 | 292 ± 25 | 217 ± 19 | 13.5 ± 0.9 (10) | - | - | - |
| Locke’s/Locke’s | 0.82 ± 0.09 (13) | 161 ± 5 | 324 ± 22 | 235 ± 19 | 16.9 ± 0.8 (22) | 39.0 ± 1.8 (15) | 124 ± 1 | 159 ± 17 (7) |
| Ames’/Ames’ | 1.04 ± 0.06 (19) | 181 ± 6 | 369 ± 18 | 265 ± 17 | 15.1 ± 0.6 (50) | 32.6 ± 2.2 (19) | 127 ± 4 | 376 ± 23 (23) |
| Ames’Ca,Mg/Ames’Ca,Mg | 0.90 ± 0.10 (20) | 187 ± 6 | 332 ± 24 | 230 ± 22 | 14.5 ± 0.7 (29) | 34.4 ± 1.6 (23) | 125 ± 3 | 173 ± 10 (15) |
| Ames’Mg/Ames’Mg | 0.60 ± 0.05 (30) | 190 ± 5 | 318 ± 13 | 217 ± 10 | 13.5 ± 0.5 (33) | 33.8 ± 3.5 (23) | 124 ± 7 | 209 ± 10 (16) |