Table 2.
Summary of microarray and qPCR data from the acute cocaine experiment.
| Gene symbol | Description | Gene ID | Microarray | Quantitative PCR |
|---|---|---|---|---|
| Fos | c-fos; FBJ osteosarcoma oncogene; Immediate early gene | 314322 | +2.7 fold | F(2,9)=1.887, p=0.207 βgal+ vs βgal−: p=0.229 βgal+ vs NeuN+: p=0.09 βgal− vs NeuN+: p=0.446 |
| FosB | fra-2; FBJ osteosarcoma oncogene B; Immediate early gene | 308411 | --- | F(2,11)=5.520, p<0.05 βgal+ vs βgal−: +6.3 fold; n=5,6; p<0.05 βgal+ vs NeuN+: +7.5 fold; n=5,3; p<0.05 βgal− vs NeuN+: p=0.938 |
| Arc | Activity-regulated cytoskeleton-associated protein | 54323 | +4.6 fold | F(2,8)=17.402, p<0.005 βgal+ vs βgal−: +6.6 fold; n=4,4; p<0.005 βgal+ vs NeuN+: +3.4 fold; n=4,3; p<0.005 βgal− vs NeuN+: p=0.419 |
| Nr4a3 | Nuclear receptor subfamily 4, group A, member 3; transcription factor | 58853 | +5.2 fold | F(2,9)=11.456, p<0.005 βgal+ vs βgal−: +8.0 fold; n=4,5; p<0.005 βgal+ vs NeuN+: +5.1 fold; n=4,3; p<0.01 βgal− vs NeuN+: p=0.742 |
| Pdyn | Prodynorphin; peptide found in D1 expressing striatal neurons | 29190 | +2.3 fold | F(2,6)=70.047, p<0.001 βgal+ vs βgal−: +3.6 fold; n=3,3; p<0.001 βgal+ vs NeuN+: +3.1 fold; n=3,3; p<0.001 βgal− vs NeuN+: p=0.515 |
| Adora2a | Adenosine A2a receptor; found in D2 expressing striatal neurons | 25369 | −3.0 fold | F(2,8)=2.690, p=0.128 βgal+ vs βgal−: p=0.204 βgal+ vs NeuN+: p=0.051 βgal− vs NeuN+: p=0.342 |
| Kcnc1 | Kv4; Kv3.1; Potassium voltage gated channel, Shaw-related subfamily, member 1 | 25327 | −2.6 fold | F(2,9)=16.942, p<0.005 βgal+ vs βgal−: −2.2 fold; n=4,5; p<0.01 βgal+ vs NeuN+: −3.1 fold; n=4,3; p<0.001 βgal− vs NeuN+: −1.4 fold; p=0.031 |
| Map2k6 | Mkk6; Mitogen-activated protein kinase kinase 6; activator of p38 MAPK | 114495 | −5.2 fold | F(2,9)=17.196, p<0.005 βgal+ vs βgal−: −7.9 fold; n=4,5; p<0.05 βgal+ vs NeuN+: −15.1 fold; n=4,3; p<0.001 βgal− vs NeuN+: −1.9 fold; p=0.013 |
One way ANOVA (F and p values are shown in Quantitative PCR column) was used to compare gene expression in three groups of cells: βgal-positive neurons and βgal-negative neurons from the same rats after a single injection of cocaine, and all neurons (NeuN-positive; NeuN+) from different rats after a single injection of saline. Fold-level changes are relative to control levels in NeuN+ cells. Positive fold-level changes indicate higher levels while negative fold-level changes indicate lower levels than in NeuN+ control samples.