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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: J Neurosci. 2006 Apr 19;26(16):4166–4177. doi: 10.1523/JNEUROSCI.0176-06.2006

Figure 8.

Figure 8

Non-postsynaptic NMDA receptors are required for t-LTD and anandamide-induced synaptic depression. A, Intermediate duration (10–15 min) D-AP5 (50 μM) does not block t-LTD, but long-duration (20–33 min) D-AP5 does block t-LTD. Long-duration (24–26 min) internal MK-801 does not block t-LTD. This indicates that postsynaptic NMDA receptors are not the source of slow modulation of t-LTD. B, Bath-applying D-AP5 for 20 min or longer blocks anandamide (AEA)-induced synaptic depression, relative to interleaved control cells with no D-AP5. C, D-AP5 does not block synaptically evoked AMPA receptor currents measured in voltage clamp at −90 mV in single pulses at 0.1 Hz (top) but does block currents when seven pulses are evoked at 50 Hz (bottom). Insets, Single examples of AMPA currents before (black) and after D-AP5 (gray). D, D-AP5 does block AMPA currents measured at −90 mV in trains of two pulses at 30 Hz in the presence of 25 μM TBOA. Inset, Single example of AMPA currents before (black) and after D-AP5 (gray). E, Summary of effects of D-AP5 on the amplitudes (Ampl.) of the first and second EPSCs at 0.1, 30, and 50 Hz and 30 Hz in the presence of TBOA, under normal conditions (control), and in the presence of internal MK-801 (iMK-801). Asterisks indicate significance from baseline using a paired t test.