Table 1.
zCCN mRNA expression during embryonic development detected by in situ hybridization
| Shield | 80% epiboly | 5–8 somite | 24 hpf | 48 hpf | 50–56 hpf | 70 hpf | 96 hpf | 120 hpf | |
|---|---|---|---|---|---|---|---|---|---|
| Cyr61-c5 | − | − | − | + | + | + | + | − | − |
| Cyr61-c8 | − | − | + | − | − | − | − | − | − |
| Cyr61-c23 | − | + | + | + | + | − | + | + | + |
| CTGF-c19 | − | − | + | + | + | + | + | + | + |
| CTGF-c20 | − | − | + | + | + | + | + | + | + |
| WISP1-c16 | − | − | − | − | − | + | − | − | − |
| WISP1-c19 | − | − | − | − | − | − | − | − | − |
| WISP2-c23 | − | − | − | − | − | − | + | − | − |
| WISP3-c20 | − | − | + | + | + | + | + | + | + |
Greater than 20 embryos were examined for all antisense probes, except for 50–56 hpf; at this time point only WISP1-c16 was assayed in more than 20 embryos. Control hybridizations used sense probes for each zCCN and gave no signal.