Figure 4. Abolished CHS response in CD11c-TAK1^fl/fl mice.

(A) On Day1, CD11c-TAK1^fl/fl and CD11c-TAK1^fl/wt mice were painted with DNFB on the de-haired back skin. Mice were then challenged with DNFB on Day 6. Before (open bar) and 48-hour post (solid bar) challenge, ear thickness was measured and plotted (top panel). The changes of ear thickness (Δear thickness) were also calculated and plotted (lower panel). Mean values of four mice ±SD are shown and the P value is indicated. (B) CD11c⁺, CD4⁺ cells were purified from CD11c-TAK1^fl/wt mice. PCR analysis for floxed-TAK allele (TAK1^fl) and wt TAK1 allele (TAK1^wt) (served as internal control) were performed.