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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: J Neurosci. 2011 Mar 30;31(13):4886–4895. doi: 10.1523/JNEUROSCI.5122-10.2011

Figure 5. Overexpression of otoferlin in chromaffin cells does not change kinetics or amount of exocytosis and does not restore synchronous exocytosis in Syt1 deficient cells.

Figure 5

A, TIRF image of the footprint of a chromaffin cell, which had been transfected with a otoferlin-eGFP fusion construct. Individual fluorescent spots most probably represent otoferlin-eGFP-tagged chromaffin granules, scale bar: 1 µm.

B, average ΔCm in response to the first flash in control (n = 20; black) and otoferlin-expressing (n = 20; red) bovine chromaffin cells. The first flash was delivered 120–180 s after establishment of the whole-cell configuration.

C, exocytic responses of a wild-type non-transfected mouse chromaffin cell (black) and mean response of 5 Syt1 deficient chromaffin cells that had been transfected to express the otoferline-GFP fusion construct: lack of the fast component of the exocytic burst. Insert: representative exocytotic response from wild-type (black, postflash [Ca2+]i: 16.31 µM) and Syt1 knock-out (red, postflash [Ca2+]i: 15.5 µM).