Ionotropic glutamate receptors, group I mGluRs, group III mGluRs, acetylcholine receptors and GABAB receptors are not involved in the regulation of developmental increase in neuronal gap junction coupling. Pharmacological manipulations (see text for details and concentrations) were performed in rat hypothalamic cultures on DIV3-15 followed by assessment of Cx36 protein expression on DIV15. Optical density signals are normalized relative to tubulin, and normalized values are compared to the control (set at 1.0). Statistical data are presented: paired Student’s t-test relative to control; mean ± SEM; n = 6 in each group. No significant difference between the control and indicated treatments was found. A/C, AP5 plus CNQX; Atr/Mec, atropine plus mecamylamine.