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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Nat Commun. 2011 Feb;2:187. doi: 10.1038/ncomms1187

Figure 1. Brg1 is required for early mouse heart formation.

Figure 1

(a) Activity of the Nkx2.5::Cre transgene, using the Z/EG or RYR reporter, at E8.5, 9.5, 11 and 12.5. Inset for E9.5 embryo shows a ventral four-chamber view. For whole-mount pictures, green signal is the activity of the Z/EG reporter, whereas red signal is the bright-field illumination through a red filter. For the RYR reporter, a cryosection stained for anti-EYFP (green), alpha-tropomyosin (red) and 4,6-diamidino-2-phenylindole (blue) is shown. Original magnification: ×25 (whole-mount pictures), ×100 (sections). (b) Frontal view of OPT reconstructions (left panels), lateral view of OPT reconstructions (middle panels) and histology (rightmost panels) of WT and Brg1 mutant (Nkx2.5:: Cre;Brg1f/f) mice at E9.5. Arrowhead shows thickened ventricular wall. (c) Histology of postnatal day (P) 1 hearts. Arrow shows membranous ventricular septal defect and double outlet right ventricle in the Nkx2.5::Cre;Brg1f/f heart. Close-up of the interventricular septum (right panels) shows disorganized septum formation. Original magnification: ×100. (d) Gene expression in WT and Nkx2.5::Cre;Brg1f/f mice at E8.5 (Actc1) or E9.5 (all other genes) shows decreased Tbx5, Nppa, Bmp10 and Hand1 expression. la, left atrium; lb, limb bud; lv, left ventricle; ra, right atrium; rv, right ventricle; v, ventricle; v?, ventricle of ambiguous identity.