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Representative example of flow cytometry analysis of thymocytes stained with anti-CD4, anti-CD8, anti-CD25, and anti-Foxp3 antibodies revealed that generation of nTregs is preserved in the thymus of 4 to 5-weeks-old lig4R/R and rag1S/S mice as compared to what observed in WT age-matched littermates. At least six mice per group were analyzed.
