Table 1. Number of c-Fos positive neurons in Alcw2 congenic and D2 background strain mice.
Brain regional numbers of c-Fos-positive neurons in Alcw2 congenic and background strain mice. Congenic and background strain (D2) mice were administered 4 g/kg ethanol (n=14 and 16, respectively) or saline control (n=5 per strain). Seven hours later, the brains were removed and processed for immunohistochemical analysis. Values represent the number of c-Fos immunoreactive neurons (mean ± SEM) in representative sections. Significant effects of treatment (T) and strain (S), based on the results of Kruskal Wallis one-way ANOVA, are indicated.
| Congenic
|
D2
|
ANOVA | |||
|---|---|---|---|---|---|
| Saline | Ethanol | Saline | Ethanol | ||
| Extended basal ganglia | |||||
| Dorsomedial striatum | 0 ± 0 | 6 ± 2 | 0 ± 0 | 13 ± 4 | T |
| Dorsolateral striatum | 0 ± 0 | 9 ± 2 | 0 ± 0 | 14 ± 2 | T |
| Nucleus accumbens, shell | 43 ± 9 | 83 ± 9 | 21 ± 9 | 97 ± 10 | T |
| Nucleus accumbens, core | 17 ± 5 | 59 ± 8 | 8 ± 3 | 65 ± 9 | T |
| Lateral globus pallidus (LGP) | 0 ± 0 | 114 ± 24 | 0 ± 0 | 192 ± 31 | T |
| rostromedial LGP | 0 ± 0 | 80 ± 19 | 0 ± 0 | 156 ± 17 | T, S |
| Medial globus pallidus (MGP) | 1 ± 1 | 86 ± 16 | 1 ± 1 | 157 ± 18 | T |
| medial MGP | 1 ± 0 | 51 ± 10 | 0 ± 0 | 85 ± 12 | T |
| Ventral pallidum (VP) | 0 ± 0 | 98 ± 19 | 0 ± 0 | 158 ± 20 | T, S |
| Substantia nigra, reticulata (SNr), rostral | 1 ± 1 | 131 ± 32 | 1 ± 1 | 242 ± 25 | T, S |
| SNr, caudal | 2 ± 1 | 227 ± 41 | 2 ± 1 | 326 ± 19 | T, S |
| dorsomedial SNr | 1 ± 0 | 53 ± 12 | 0 ± 0 | 85 ± 11 | T, S |
| Substantia nigra, compacta (SNc) | 0 ± 0 | 9 ± 5 | 0 ± 0 | 13 ± 4 | T |
| Subthalamic nucleus (STN) | 0 ± 0 | 183 ± 32 | 0 ± 0 | 302 ± 28 | T, S |
| medial STN | 0 ± 0 | 101 ± 20 | 0 ± 0 | 149 ± 14 | T |
| Extended Amygdala | |||||
| Central nucleus (CEA) | 23 ± 5 | 62 ± 11 | 5 ± 2 | 59 ± 12 | T |
| Basolateral amygdala (BLA) | 20 ± 7 | 54 ± 9 | 14 ± 4 | 56 ± 10 | T |
| Bed nucleus of the stria terminalis | |||||
| (BNST) | 7 ± 2 | 51 ± 9 | 2 ± 1 | 59 ± 8 | T |
| Septum | |||||
| Lateral septum | 42 ± 8 | 46 ± 8 | 39 ± 13 | 49 ± 9 | |
| Medial septum | 6 ± 2 | 11 ± 2 | 3± 2 | 9 ± 1 | T |
| Cortex | |||||
| Cingulate, area 1 (Cg1) | 25 ± 3 | 45 ± 8 | 20 ± 7 | 53 ± 7 | T |
| Prelimbic (PrL) | 58 ± 8 | 100 ± 12 | 46 ± 11 | 108 ± 13 | T |
| Ectorhinal-perirhinal cortex (Ect-PRh) | 38 ± 2 | 166 ± 9 | 39 ± 6 | 170 ± 12 | T |
| SNr outputs | |||||
| Ventromedial thalamus | 0 ± 0 | 64 ± 18 | 1 ± 1 | 91 ± 14 | T |
| Superior colliculus, intermediate layers (SCi) | 78 ± 18 | 109 ± 26 | 27 ± 9 | 164 ± 28 | T |