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. Author manuscript; available in PMC: 2011 Jun 17.
Published in final edited form as: Sensors (Basel). 2011 Mar 21;11(3):3327–3355. doi: 10.3390/s110303327

Figure 6.

Figure 6

Quantitative and qualitative HRP activity measurements on PS-b-PVP nanodomains. (A) Control experiment without HRP molecules on PS-b-PVP. (B) (1) Assay carried out with HRP molecules on PS-b-PVP. AFM panels in (A) and (B) are 180 × 180 nm in scan size. (C) (A) No absorbance peaks from the control experiment involving only the PS-b-PVP template. (B) Characteristic UV/VIS absorbance peaks were monitored due to HRP bound on PS-b-PVP. (D). UV/VIS absorbance of HRP molecules monitored over time in solution (data shown in blue) and on PS-b-PVP micelles (data shown in red). When compared to the activity of HRP molecules in solution, HRP molecules bound on PS-b-PVP showed 78% of the activity. Adapted with permission from [100].