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. Author manuscript; available in PMC: 2011 Aug 5.
Published in final edited form as: World J Oncol. 2011 Apr;2(2):53–63. doi: 10.4021/wjon301w

Figure 4.

Figure 4

Changes expression of pro-apoptotic and anti-apoptotic proteins in Ewing’s sarcoma xenografts. Treatments for 8 or 15 days: CTL, 4-HPR (20 μg/kg/day), GST (2 mg/kg/day), and 4-HPR (20 μg/kg/day) plus 4-h later GST (2 mg/kg/day). Representative Western blots (n ≥ 3) showed expression of (A) 42 kD β-actin, 25 kD Smac/Diablo, 72 kD BIRC-2, 68 kD BIRC-3, 20 and 12 kD active caspase-3, and 67 kD AIF in SK-N-MC xenografts; and (B) 42 kD β-actin, 65 kD NF-κB, 21 kD VEGF, 17 kD FGF2, and 32 and 20 kD caspase-3 in RD-ES xenografts. (C) Colorimetric assay for determination of caspase-3 activity in RD-ES xenografts. Significant difference between CTL and a treatment was indicated by *P < 0.05 or **P < 0.001.