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. Author manuscript; available in PMC: 2011 Aug 5.
Published in final edited form as: World J Oncol. 2011 Apr;2(2):53–63. doi: 10.4021/wjon301w

Figure 5.

Figure 5

In situ single and double immunofluorescent labelings to detect increases in expression of calpain and caspase-12 during apoptosis (TUNEL-positive cells) in SK-N-MC xenografts. Treatments for 8 days: CTL, 4-HPR (20 μg/kg/day), GST (2 mg/kg/day), and 4-HPR (20 μg/kg/day) plus 4-h later GST (2 mg/kg/day). (A) Combination therapy most significantly increased calpain expression in SK-N-MC xenografts. (B) Calpain expression in TUNEL-positive cells. (C) Combination therapy most significantly increased caspase-12 expression in SK-N-MC xenografts. (D) Caspase-12 expression in TUNEL-positive cells. In bar graphs: 1 = CTL, 2 = 4-HPR, 3 = GST, and 4 = 4-HPR plus GST. Significant difference between CTL and a treatment was indicated by *P < 0.05 or **P < 0.001.