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. Author manuscript; available in PMC: 2007 Dec 20.
Published in final edited form as: J Neurosci. 2005 Jan 19;25(3):748–757. doi: 10.1523/JNEUROSCI.2399-04.2005

Figure 4.

Figure 4

Expressing PRPF31–GFP protein in primary retinal cells. Primary retinal cell cultures were established as described in Materials and Methods. A calcium-phosphate method was established to transfect primary retinal cells. Twelve hours after transfection, cells were fixed and immunostained using anti-rhodopsin antibody, and images were captured. A phase-contrast image (A), rhodopsin immunostaining signal (B), a superimposed A–B image (C), nuclear staining (D), green fluorescence (E), and a superimposed B–D–E image (F) are shown. The arrowheads mark two cells that are rhodopsin negative. PRPF31–GFP-expressing cells are marked by white arrows (E, F). Nuclear morphology as detected by bis-benzimide (D), GFP fluorescence (E), and superimposition of B, D, and E (F) demonstrate that our transfection method did not cause nonspecific cytotoxicity and allowed us to express PRPF31–GFP in rhodopsin-positive retinal cells.