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. Author manuscript; available in PMC: 2007 Dec 20.
Published in final edited form as: J Neurosci. 2005 Jan 19;25(3):748–757. doi: 10.1523/JNEUROSCI.2399-04.2005

Figure 7.

Figure 7

Mutant PRPF31 proteins do not affect synapsin expression. Primary retinal cells were transfected with plasmids expressing either wild-type or mutant PRPF31 as GFP-fusion proteins; wild-type (A1–A4), N371 (B1–B4), or N256 (C1–C4) PRPF31 proteins were detected in the transfected primary retinal cells by monitoring GFP fluorescence (A3–C3). Synapsin expression was detected by immunofluorescent staining (red fluorescence) using a monoclonal anti-synapsin antibody followed by the staining with the secondary antibody conjugated with Cy3 (A2–C2). The nuclear staining was performed with bis-benzamide (A4 –C4). A1–C1 show the superimposed images in columns 2– 4. The synapsin expression in cells expressing mutant PRPF31–GFP proteins shows no significant difference from those expressing the wild-type PRPF31–GFP protein (compare B2 and C2, with A2).