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. Author manuscript; available in PMC: 2007 Nov 1.
Published in final edited form as: J Biol Chem. 2007 Mar 22;282(21):15349–15356. doi: 10.1074/jbc.M700156200

FIGURE 8. Effect of IHPK2 mutation on IFN-β antiproliferative activity.

FIGURE 8

NIH-OVCAR-3 cells were stably transfected with IHPK2 constructs and grown in the presence of 5–500 units/ml IFN-β. After 4 days, cells were fixed and stained with sulforhodamine B. Absorbance of bound dye was expressed as percent of untreated controls (n = 8, each data point). Overexpression of IHPK2 sensitized cells to IFN-β. Cells expressing IHPK2 mutations S3&4 in combination conferred relative resistance to IFN-β. The dose response curve of cells expressing irrelevant IHPK2 mutations (S1, S2, S3, S4, S1&2, S1&3, S1&4, S2&3, S2&4, S1&2&3, S1&2&4) lay in a narrow band between pCXN2 (vector) and IHPK2 and is represented as a single curve (IRREL).