Table 2.

Summary of PCR results of oral fluids (OF) and nasal swabs (NS) from contact and seeder pigs and virus isolation of oral fluids (shaded cells) by collection day, replica, and treatment (each room had 1 seeder pig and 10 contacts).

Group	Room		Sample	Days posteontact
				0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
Control	1	Contacts	NS	0*	0	3	7	9	10	10	1	3 susp†	NT‡	NT	NT	NT	NT	NT	NT
		Seeder	NS		1	1	1	1	0	0	0	0	NT	NT	NT	NT	NT	NT	NT
			OF	neg	pos¥	pos	pos	pos	pos	pos	pos	neg	NT	NT	NT	NT	NT	NT	NT
	2	Contacts	NS	0	3	9	10	10	10	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
		Seeder	NS		1	1	1	1	0	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
			OF	neg	pos	pos	pos	pos	pos	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
Heterologous	1	Contacts	NS	0	0	0	0	0	1	1	1	1	1 susp	0	0	0	0	0	0
		Seeder	NS		1	1	1	1	0	0	0	0	0	0	0	0	0	0	0
			OF	neg	pos	pos	pos	neg	pos	pos	pos	pos	neg	neg	neg	neg	neg	neg	neg
	2	Contacts	NS	0	0	3	5	5	5	4	2	0	1 susp	0	0	0	0	0	NT
		Seeder	NS		1	1	1	0	0	0	0	0	0	0	0	0	0	0	NT
			OF	neg	pos	pos	pos	pos	pos	pos	neg	neg	neg	neg	neg	neg	neg	neg	NT
	3	Contacts	NS	0	0	0	0	0	0	0	1	1	1	1	2	1	0	0	NT
		Seeder	NS		1	1	1	1	0	0	0	0	0	1	0	0	0	0	NT
			OF	neg	pos	pos	pos	neg	neg	neg	suspect	pos	pos	pos	suspect	neg	neg	neg	NT
Homologous	1	Contacts	NS	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
		Seeder	NS		1	1	1	1	0	0	0	0	0	0	0	0	0	0	0
			OF	neg	suspect	pos	pos	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg
	2	Contacts	NS	0	0	0	0	0	0	0	0	0	0	0	0	0	0	NT	NT
		Seeder	NS		1	1	1	1susp	1susp	0	0	0	0	0	0	0	0	NT	NT
			OF	neg	pos	pos	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	NT	NT
	3	Contacts	NS	0	0	0	0	0	0	0	0	0	0	0	0	0	0	NT	NT
		Seeder	NS		1	1	1susp	0	0	0	0	0	0	0	0	0	0	NT	NT
			OF	neg	pos	pos	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	neg	NT	NT

^*Number of positives

^†Results classified as 'suspect' by RRT-PCR (35–40 CTs) were considered negative for statistical analysis

^‡NT: Non-tested, groups were euthanized when all pigs became positive (controls) or at the termination of the study between 13 to 15 days

^¥Shaded cells indicate virus isolation results from oral fluid samples