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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Mol Oncol. 2011 Jul 26;5(5):426–437. doi: 10.1016/j.molonc.2011.07.004

Figure 5. Multiple transcription factors are involved in the regulation of p27 in cathepsin B- and uPAR-depleted glioma cells.

Figure 5

4910 and 5310 cells were initially transfected with SV or pCU or treated with U0126 (10 μM) or Wortmannin (10 μM). 24 hrs after treatment, a second transfection with the luciferase constructs was performed as described in Materials and Methods. Luciferase expression was quantified using Promega's Luciferase Assay Kit with a Turner Luminometer and is represented graphically. The graphs show luciferase expression when p27-1-luc (A) and p27-2-luc (B) constructs were used. Assessment for luciferase expression was performed at least in triplicate (*p<0.05, **p<0.01). At the bottom of each graph, the effect of pCU treatment on the expression of respective transcription factors is shown. C. After treating for 24 hrs with 10074-G5 (c-Myc Max interaction inhibitor) cell lysates were subjected to immunoblot analysis to check the expression of p27, c-Myc and Max. GAPDH was used as loading control. Immunoprecipitation analysis of Max and FOXO3a after pull down with c-Myc in untreated and 10074-G5-treated cell lysates (bottom panel).