A, The activation of the IM in NPY neurons was recorded from ovariectomized, oil-treated (n=14); E2-treated (n=12) and fasted, E2-treated (n=11) female mice using the protocol described in Figure 3. The current at +10 mV for the three treatments was 231 ± 24 pA; 513 ± 82 pA; and 250 ± 31 pA, respectively. I–V plots were analyzed by a two-way ANOVA (p<0.001, F=11.0, df=2) followed by Bonferroni-Dunn comparison. a, p<0.05; b, p<0.01; c, p<0.001 compared to E2-treated. B, A plot of the conductances at 0 mV between each steroid treatment comparing control, XE991-sensitive and -insensitive currents. C, Summary of the ratio ((Inst-SS)/SS) of the time-dependent (Inst-SS) and steady state (SS) currents from control and XE991-treated conditions in response to a +10 mV step. *, p<0.05; **, p<0.01.