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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: J Neurosci. 2011 Aug 17;31(33):11825–11835. doi: 10.1523/JNEUROSCI.1395-11.2011

Figure 8. 17β-estradiol regulation of KCNQ5 expression is ERα–dependent and a small population of NPY neurons express ERα.

Figure 8

A, E2 treatment in ovariectomized, wild-type females increased the mRNA expression of KCNQ5 in the arcuate nucleus but failed to regulate KCNQ5 expression in ERα knock-out mice. *, p<0.05. The number in the column equals the number of animals per treatment. B, A representative gel illustrating the expression of ERα in NPY neurons harvested from ovariectomized females and the co-expression of KCNQ5. C, Immunocytochemistry showed a small population of GFP-NPY neurons co-localizing ERα: NPY-GFP neurons to left and ERα-immunoreactive neurons in middle with an overlay illustrating co-localization (indicated by arrows) to right. 99% of GFP-labeled neurons express NPY mRNA. 3V demarks the third ventricle. White bar in the overlay represents 25 µm.