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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: J Neurosci. 2011 Aug 17;31(33):11762–11771. doi: 10.1523/JNEUROSCI.2707-11.2011

Figure 4. Critical role of TrkB activation within the first 40 min of L-LTP, but not in STP.

Figure 4

(A) 1NMPP1 blocked 12TBS-induced TrkB activation in TrkBF616A mice. The CA1 regions of hippocampal slices from TrkBF616A mice were microdissected 1 hour after 12TBS. For each experiment, 3 mice were used, and for each condition, 4 CA1 slices were used. The same experiment was repeated 5 times (n = 5), each with new batch of animals. 1NMPP1 (5 µM), but not vehicle control (DMSO), blocked the 12TBS-induced increase of pTrkB in TrkBF616A mice (*, P <0.003; one-way ANOVA).

(B) Blockade of 12TBS induced L-LTP by inhibiting TrkB activation with 1NMPP1 in adult hippocampal slices derived from TrkBF616A mice. 1NMPP1 (5 µM), applied to slices throughout the experiment, completely blocked L-LTP (blue diamonds), while vehicle control had no effect (black circles). 1NMPP1 did not affect L-LTP in the control mice (C57BL/6) (pink diamonds).

(C) 1NMPP1 applied immediately after 12TBS also blocked L-LTP in TrkBF616A slices.

(D) Inhibition of L-LTP by 1NMPP1 applied between 0 and 40 min after 12TBS (red) but not 45 min after 12TBS in TrkBF616A slices (black).

(E) Stable baseline recording were obtained for over 4 hours without any treatment.

(F) Indistinguishable STPs in 1NMPP1- and vehicle-treated TrkBF616A slices. STPs were induced by 2TBS. In both cases, fEPSPs returned to baseline at 60 min after 2TBS.