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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Cell Res. 2011 Oct 11;22(4):746–756. doi: 10.1038/cr.2011.162

Figure 1. RU-486 is a high affinity PPARγ agonist.

Figure 1

Figure 1

(A) Receptor-specific transactivation by RU-486. Cos7 cells were cotransfected with pG5Luc reporter together with the plasmids encoding various nuclear receptors LBDs fused with the Gal4 DNA-binding domain. After transfection, cells were treated with DMSO or 1 μM RU-486. (B) Dose responses of RU-486 in transactivating PPARγ. Cos7 cells were cotransfected with plasmids encoding full-length PPARγ and a PPRE luciferase reporter. After transfection, cells were treated with DMSO or various concentrations of RU-486 and rosiglitazone. (C) PPARγ activation by RU-486 was inhibited by PPARγ antagonist GW9662. Cos7 cells were cotransfected with plasmids encoding full-length PPARγ and a PPRE luciferase reporter. After transfection, cells were treated with RU-486 together with various concentrations of GW9662.