S1P1 receptor is expressed on pulmonary endothelium and lymphocytes but not on pulmonary epithelial cells. Flow cytometry histograms showing eGFP fluorescence from heterozygous S1P1-eGFP on lung endothelial and epithelial cells mice, (A) CD4, CD8 T cells and B cells (B) and macrophages, neutrophils, dendritic cells and NK cells (C), prior to influenza virus infection. (D) Relative expression of S1P1-eGFP on lung cell populations before or 48-hours after influenza virus infection. The numbers in the upper right corner of each plot represents the relative eGFP expression as calculated by dividing the MFI of eGFP on S1P1-eGFP transgenic mice over eGFP MFI of C57BL/6J littermate controls. Data represent average SD from 2–5 mice/group. Results are representative of 3 independent experiments.