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In vitro calcification is attenuated in S100A12tg aortic SMCs by pretreatment with soluble RAGE and by transfection with shRNA targeted to Nox1. a) S100A12, RAGE and Nox-1 protein expression in aortic tissue from WT and S100A12tg mice, b) calcium content and c) mRNA in cultured aortic SMC form S100A12tg and WT aortas. Modified and reproduced with permission from S.Karger AG, Basel [19].
