Table 3.
βII-C Interacting Proteins (IPβII-C s) and Their Effects on Tetramerization Site Interaction
| IPβII-C | Matching Sequence |
Homo sapiens Proteins in Databases (Accession #), Matching Fragment |
Difference | αII-Na | Effect on Tetramerb |
|---|---|---|---|---|---|
| 7 | 1-247 (247)c | THAP domain containing, apoptosis associated protein 3, isoform CRA g (EAW71568), residues 38-284 (284)c |
0% | yes | no |
| 10 | 1-260*d (260) | Spectrin, beta, non-erythroid (Q01082), residues 1781-2040 (2314) *G20E, F235Sd |
1% | yes | no |
| 2 | 6-483 (483) | Glioma tumor suppressor candidate region gene 2 (NP_056525), residues 1-478 (478) |
1% first 5 aa (SSFDK) |
yes | no |
| 12 | 1-369* (369) | Septin 8 isoform c (NP_001092282), residues 74-442 (442) | 1% *H239Q, P344T, F362S |
yes | no |
| 9 | 1-250* (250) | Coatomer protein complex, subunit beta 1 (NP_057535), residues 704-953 (953) |
1% *Y53C |
no | not tested |
| 4 | 1-271 (278) | Zinc-finger protein 251 (NP_612376), residues 146-416 (671) | 2% last 7 residues |
yes | no |
| 1 | 1-145 (153) | Syntaxin binding protein 1 (NP_003156), residues 284-428 (603) |
4% last 8 residues |
no | yes |
| 3 | 6-263, 288-483 (483) |
Glioma tumor suppressor candidate region gene 2 (NP_056525), residues 1-258; 283-478 (478) |
6% first 5 aa (SSFDK) |
yes | no |
| 8 | 19-131 (131) | Ubiquitin-conjugating enzyme E2L3 (BAG61806), residues 100-212 (212) |
14% first 18 aa |
no | not tested |
| 6 | 1-247*e (303) | Golgin A6 family-like 10 (NP_001157937), (479) | 26% | yes | no |
| 5 | 32-386* (386) | Zinc-finger protein 251 (NP_612376), residues 317-671 (671) | 27% (*numerous mutations) |
yes | no |
| 11 | 4-79 (124) | Eukaryotic translation initiation factor 3, subunit H (EAW91959), residues 1-76 (332) |
30% (*numerous mutations) |
yes | no |
| 13-17 | Unknowns 1, 2, 3, 4 | yes | no |
a
effects of αII-N on IPβII-C and βII-C interaction, with “yes” indicating that αII-N abolishes IPβII-C interaction with βII-C;
b
effects of IPβII-C on αII-N and βII-C interaction;
c
total number of residues in protein;
d
the symbol * indicates mutations exist.
e
IPβII-C res 1-150 correspond to res 47-196 with 5 mutations (I4V, E39Q, R156H, R163C, Q169L, D174E, R191C), res 151-168 correspond to res 211-228 and res 169-247 correspond to res 243-321 with E237D.