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. Author manuscript; available in PMC: 2011 Sep 28.
Published in final edited form as: Mol Microbiol. 2011 Jul 18;81(5):1313–1329. doi: 10.1111/j.1365-2958.2011.07765.x

Figure 8. LPS from vc0212 deletion mutant does not stimulate innate immune recognition by human TLR4-MD2.

Figure 8

HEK-293 cells transfected with hTLR4-MD2 and co-receptor hCD14 were treated overnight using 10-fold dilutions of highly purified LPS isolated from the indicated strains of V. cholerae. Additionally, LPS from R. sphaeroides and E. coli was utilized as negative and positive controls, respectively. Values are the mean of results from triplicate wells ± standard deviation. LPS isolated from the wild type V. cholerae O1 El Tor was found to stimulate TLR4-dependent NF-κB activation in HEK-cells, similarly to the positive control. However, LPS isolated from the vc0212 deletion mutant did not elicit TLR4-dependent NF-κB activation. LPS isolated from the vc0212 deletion mutant complemented with either vc0212 or the Y. pestis acyltransferase fully restored TLR-4 stimulation of NF-κB. Wild type LPS and LPS isolated from complemented strains at 1.0–1,000 ng/ml showed a significant increase (P ≤ 0.006) in hTLR4-MD2 activation of NF-κB when compared to LPS isolated from the vc0212 deletion mutant.