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. Author manuscript; available in PMC: 2011 Oct 4.
Published in final edited form as: J Leukoc Biol. 2008 May 1;84(1):115–123. doi: 10.1189/jlb.1207835

Fig. 6.

Fig. 6

Neutralization of IL-10 and TGF-β1 abrogates the inhibitory effect of α-SP-R210n on M. tuberculosis-induced production of IFN-γ. PBMC were cultured in medium alone or with 2.5 μg/ml heat-killed M. tuberculosis in the absence or presence of different IgG (10 μg/ml) as indicated. In some cases, 1 μg/ml neutralizing antibodies to IL-10 and TGF-β1 were also added. After 48 h incubation, IFN-γ levels in the culture supernatants were measured by ELISA (A), cells were collected, and IFN-γ mRNA expression was measured by real-time PCR, normalized to 18S rRNA content (B). The mean ± SE of five (A) or six (B) donors is shown.