Figure 1. RGMb deletion does not alter development of neurons as revealed by in situ hybridization.
(A) RGMb is widely expressed in small and large DRG neurons of adult C57BL/6 mice as demonstrated by co-expression with the sensory neuron markers peripherin, TrkA and neurofilament 200 (NF-200), visualized by fluorescent immunohistochemistry (right panel) and in situ hybridization (left panel). Arrows indicate cells that express peripherin, TrkA, or NF-200(white arrows) and RGMb (yellow arrows). Scale bar, 100µm. (B) In situ hybridization revealed no difference between RGMb KO and WT LM in the expression of a variety of key markers of sensory neurons in P0 DRG.