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Effect of SNL on serotonin-immunoreactivity (5-HT-ir) in the superficial dorsal horn of the spinal cord. A–C. SNL significantly decreased the density of 5HT-ir varicosities at all spinal levels examined. (A) Segment C2, (B) Segment T2, (C) Segment L4 (*, p<0.05; 2-way ANOVA). D. Images of 5-HT-ir in superficial dorsal horn of rats receiving sham surgery or SNL. Note reduced 5-HT-ir in SNL image. Box in inset denotes area from which images were obtained.
