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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Plant Pathol. 2012 Feb 9;13(7):677–689. doi: 10.1111/j.1364-3703.2011.00779.x

Fig. 1. MoSwi6 interacts with MoMps1.

Fig. 1

(A) A yeast two-hybrid assay was used to examine the interaction between MoSwi6 as a bait and MoMps1 as a prey. The interaction between pGBKT7-53 and pGADT7-T was used as the positive control, and non-interactions between pGBKT7-Lam and pGADT7-T, BD (pGBKT7)-MoSwi6 and AD (pGADT7), BD and AD-MoMps1, and empty AD and BD vectors were used as negative controls. Plates were incubated at 30°C for three days before being photographed. (B) co-IP assay for the interaction of MoSwi6 with MoMps1. Western blot analysis with total proteins (Total) isolated from transformants co-expressing the MoSWI6-GFP and MoMPS1-3xFLAG constructs and proteins eluted from the anti-FLAG M2 beads (Elution). The presence of MoSwi6 and MoMps1 was detected with an anti-GFP and an anti-FLAG antibody, respectively.