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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Plant Pathol. 2012 Feb 9;13(7):677–689. doi: 10.1111/j.1364-3703.2011.00779.x

Fig. 4. Laccase activity is reduced in ΔMoswi6 that can be restored by adding CuSO4.

Fig. 4

(A) After growth for 6-day with Cu2+ on CM, melanin was restored to the ΔMoswi6 mutant, particularly on medium containing 0.5 – 1 mM CuSO4. (B) (a) The Guy11 ΔMoswi6 mutants (#3 and #5), and the reconstituted (ΔMoswi6/MoSWI6) were incubated on a CM plate for 6 days and then incubated on an ABTS (0.2 mM) plate (b to d). After a 6-day treatment with 0.25, 0.5, or 1 mM CuSO4 respectively, the Guy11 ΔMoswi6 mutants (#3 and #5) and the reconstituted (ΔMoswi6/MoSWI6) were incubated on an ABTS plate (0.2 mM) for 24 hours. (e to g) The Guy11 ΔMoswi6 mutants (#3 and #5) and the reconstituted (ΔMoswi6/MoSWI6) were incubated on CM for 6 days and then incubated on an ABTS plate (0.2 mM) supplemented with 0.25, 0.5, and 1 mM CuSO4 for 24 hours. (C) Laccase activity absorption value at a wavelength of 420 nm. The laccase activity in the ΔMoswi6 (#3) mutant was reduced compared to that of the wild type Guy11. (D) Transcription of putative laccase genes in the ΔMoswi6 (#3) mutant and wild type Guy11. The relative abundance of the transcripts compared with the standard condition (wild type) is displayed as a number. Data represent three independent experiments, each performed three times.