Table 2.
Individual | Age | Adjuvant | IgA | IgE | IgG | IgM | κ light chain | λ light chain | poly IgG | poly IgM |
---|---|---|---|---|---|---|---|---|---|---|
A | 4.7 | YES | 0.464 | 0.561 | 1.623 | 2.104 | 6.2501 | 0.392 | 12.5001 | 0.722 |
B | 6.8 | NO | 0.139 | 0.138 | 0.123 | 6.361 | 4.035 | 0.077 | 5.365 | 2.502 |
C | 11.2 | YES | 0.322 | 0.350 | 0.684 | 2.361 | 6.2501 | 0.235 | 12.5001 | 1.044 |
D | 11.3 | NO | 0.042 | 0.048 | 0.024 | 0.356 | 0.824 | 0.016 | 1.365 | 0.236 |
E | 13.9 | YES | 0.011 | 0.024 | 0.009 | 0.192 | 0.371 | 0.006 | 0.820 | 0.101 |
F | 15.1 | NO | 0.003 | 0.011 | 0.002 | 0.159 | 0.106 | 0.001 | 0.322 | 0.134 |
G | 15.1 | YES | 0.034 | 0.035 | 0.018 | 0.307 | 0.608 | 0.012 | 1.183 | 0.175 |
H | 17.0 | NO | 0.010 | 0.006 | 0.001 | 0.208 | 0.055 | 0.001 | 0.160 | 0.198 |
Detection Ab catalog # | IgA5-3B | E6-71 | GPB2-2 | CM7 | CAG8-7C | FIG1-7A | 04-20-02 | 04-20-03 | ||
Detection Ab conc. (µg/ml) | 5 | 10 | 10 | 5 | 10 | 5 | 0.5 | 0.5 | ||
Serum dilution | 1:100 | 1:100 | 1:100 | 1:100 | 1:100 | 1:100 | 1:4000 | 1:1000 |
Age is in years. Concentrations are the mean of duplicate ELISA results. For all assays, the youngest four individuals had higher antibody responses than the four oldest individuals.
Indicates the concentration exceeded the linear range of the standard curve and was assigned the maximum value of the linear range of the standard curve.