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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Vet Immunol Immunopathol. 2011 Nov 2;145(1-2):110–119. doi: 10.1016/j.vetimm.2011.10.016

Table 2.

Peak anti-DNP equivalent concentration.

Individual Age Adjuvant IgA IgE IgG IgM κ light chain λ light chain poly IgG poly IgM
A 4.7 YES 0.464 0.561 1.623 2.104 6.2501 0.392 12.5001 0.722
B 6.8 NO 0.139 0.138 0.123 6.361 4.035 0.077 5.365 2.502
C 11.2 YES 0.322 0.350 0.684 2.361 6.2501 0.235 12.5001 1.044
D 11.3 NO 0.042 0.048 0.024 0.356 0.824 0.016 1.365 0.236
E 13.9 YES 0.011 0.024 0.009 0.192 0.371 0.006 0.820 0.101
F 15.1 NO 0.003 0.011 0.002 0.159 0.106 0.001 0.322 0.134
G 15.1 YES 0.034 0.035 0.018 0.307 0.608 0.012 1.183 0.175
H 17.0 NO 0.010 0.006 0.001 0.208 0.055 0.001 0.160 0.198

Detection Ab catalog # IgA5-3B E6-71 GPB2-2 CM7 CAG8-7C FIG1-7A 04-20-02 04-20-03
Detection Ab conc. (µg/ml) 5 10 10 5 10 5 0.5 0.5
Serum dilution 1:100 1:100 1:100 1:100 1:100 1:100 1:4000 1:1000

Age is in years. Concentrations are the mean of duplicate ELISA results. For all assays, the youngest four individuals had higher antibody responses than the four oldest individuals.

1

Indicates the concentration exceeded the linear range of the standard curve and was assigned the maximum value of the linear range of the standard curve.