(A) AphA protein levels in V. harveyi wild type (BB120), luxOD47E (KM83), ΔluxR (KM669), luxOD47E ΔluxR (KM812), and luxOD47E Δqrr1–5 ΔluxR (YS040). (B) AphA protein levels in V. cholerae wild type (C6706), luxOD47E (SLS340), ΔhapR (SLS390), luxOD47E ΔhapR (SLS640), and luxOD47E Δqrr1–4 ΔhapR (SLS641). Cells were harvested at OD600~1.0, and protein levels were determined using Western blot.