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. Author manuscript; available in PMC: 2012 Nov 9.
Published in final edited form as: Mol Microbiol. 2012 Jan 9;83(3):599–611. doi: 10.1111/j.1365-2958.2011.07959.x

Figure 4. Qrr sRNAs use unique pairing regions to activate aphA expression.

Figure 4

(A) RNA sequence alignment of the V. harveyi luxR, luxO mRNA with V. harveyi Qrr4 (5’ end 1–48nt) by RNAhybrid as in Figure 3A. Pairing region I and region II are colored in green and red, respectively. The qrr4 mutation in pYS121 (mutation I, denoted mut i) and pYS120 (mutation ii, denoted mut ii) are indicated below the sequences. (B)(C)(D) Fluorescence from plasmid-encoded V. harveyi LuxR-GFP (pYS141) and LuxO-GFP (pYS142) translational fusions were measured in E. coli MC4100 carrying an empty vector (pRHA109), a vector expressing a rhamnose-inducible qrr4 gene (pSTR0227), or a mutant qrr4 gene (pYS120 or pYS121). GFP from three independent cultures was measured for each strain and the means and SEMs are shown.