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. Author manuscript; available in PMC: 2012 Jun 7.
Published in final edited form as: J Neurosci. 2011 Dec 7;31(49):17835–17847. doi: 10.1523/JNEUROSCI.3297-11.2011

Figure 6. IL-10 expression specifically within the NAcc can be programmed early in development via decreased methylation of the IL-10 gene.

Figure 6

(A) Represented separately in graphical form from Figure 2, IL-10 mRNA was significantly up-regulated nearly 4-fold in handled rats when compared to non-handled control rats in the absence of morphine treatment. (B) Neonatal handling (n = 7) significantly increases the expression of IL-10 within the NAcc when compared to non-handled controls (n = 8) by postnatal day 10 (*p < 0.05). (C) Position of the CpG Island within the IL-10 gene spanning Exon 3 and Intron 3 (individual CpGs represented by each line). Primers for detection of IL-10 in immunoprecipitated (IP) and input (IN) samples were designed within this region. (D) Neonatal handling had no effect on relative methylation levels measured within whole tissue dissections of the NAcc using MeDIP assay (Handled n = 10, Control n = 9), yet (E) a significant decrease in relative methylation levels was measured in microglial cells isolated from the NAcc of handled rats (*p < 0.05), while having no effect on total IL-10 gene levels in input samples.