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. Author manuscript; available in PMC: 2012 Jan 31.
Published in final edited form as: Clin Epigenetics. 2011 Oct 26;3(3):3. doi: 10.1186/1868-7083-3-3

Figure 2. Sulforaphane decreased global DNA methylation.

Figure 2

LnCap cells were treated with SFN (15 and 30 μM) or 5-Aza-dC (5 μM) for 24 h. Genomic DNA was extracted and treated with sodium bisulfite. Global methylation status was fluorometrically quantified in a microplate-based ELISA assay. Values were derived as fold change compared to vehicle (DMSO) and represent means ± SEM, n=3. P values represent treatment values compared to vehicle.