Fig. 3.

Induction of the EhTMKB1-9 dominant negative mutant did not affect liver abscess formation: Amebae transfected with the control vector or the plasmid expressing kinase domain deletion mutant of EhTMKB1-9 were grown in presence or absence of 20 μg/ml tetracycline for 48 h prior to infection. Amebae (10⁶) were inoculated into different liver lobes of 50–60 day old Mongolian male gerbils. Induction was maintained where appropriate by adding 2 mg/ml doxycycline in the drinking water. Gerbils were sacrificed 7 days after infection, livers extracted and abscess weights measured. (A) Comparison between abscess weights formed by uninduced or induced vector transfectants (B) comparison between abscess weights formed by uninduced or induced EhTMKB9-Dn transfectants. The X-axis shows transfectant line and treatment whereas the Y-axis represents abscess weight.