Synaptotagmin expression is similar in P[sytA-D2E] mutants compared to P[sytWT] controls. A) Representative Western blots of the central nervous system of third instar larvae probed with anti-synaptotagmin and anti-actin antibodies. B) Synaptotagmin/actin ratio normalized to the mean ratio of the transgenic control, P[sytWT]. There was no significant difference between genotypes (mean ± s.e.m., p>0.3, one way ANOVA; P[sytWT] n=15, P[sytA-D2E] line #3 n=6, line #2 n=7). C) Synaptotagmin is properly localized to the larval neuromuscular junction in both mutant and control transgenic synaptotagmin lines. Scale 20 μm.