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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Brain Pathol. 2012 Mar 21;22(5):709–722. doi: 10.1111/j.1750-3639.2012.00577.x

Figure 5. Decreased white matter pathology in the spinal cord of mice immunized with KLK6 in comparison to controls at 40 days post-TMEV infection.

Figure 5

A, D Glycol methacrylate plastic-embedded sections were stained with modified erichrome/cresyl violet stain and the area of white matter pathology determined and expressed as a percent of total white matter (G). Parallel 1 mm segments of spinal cord from the same mice were embedded in paraffin and 5 µm sections stained for MBP (B and E) or for monocytes and activated microglia using a biotinylated BS-1 antibody (C and F). The spinal cord of mice immunized with Klk6 5 weeks prior to TMEV infection showed significant reductions in the percent of white matter pathology (G, ANOVA, P=0.009; *SNK P<0.05) and in the percent of spinal cord quadrants exhibiting a loss of MBP staining (H, ANOVA on ranks, P=0.03; *Dunn’s P<0.5) compared with immunization controls. Mice immunized with Klk6 prior to TMEV infection also showed fewer spinal cord quadrants associated with BS-1 positive monocytes and activated microglia (I, ANOVA, P=0.008; *SNK, P<0.05). See also Table 2.