Table 3.

Concentration of major omega-6 and omega-3 fatty acids in liver and hypothalamus phospholipids (ug/mg)

Liver- PL FA (ug/mg)	Medium fat diets (35 en% fat)			High fat diets (60 en% fat)
	1en% LA	8en% LA	8en% LA + 1en% EPA/DHA	1en% LA	8en% LA	8en% LA + 1en% EPA/DHA
18:2n-6	1.9 ± 0.2a	2.8 ± 0.2b	3.1 ± 0.2b	1.6 ± 0.1a	3.3 ± 0.2bc	3.8 ± 0.1c
20:4n-6	2.7± 0.3ac	4.7 ± 0.3b	2.7 ± 0.1a	1.9 ± 0.1c	5.0 ± 0.3b	2.9 ± 0.1a
20:5n-3	0.92 ± 0.10ac	0.13 ± 0.01b	0.87 ± 0.08a	1.07 ± 0.06ac	0.14 ± 0.01b	1.17 ± 0.05c
22:6n-3	4.2 ± 0.5bc	2.9 ± 0.2a	3.5 ± 0.2ab	3.7 ± 0.2b	3.1 ± 0.2ab	4.7 ± 0.2c
n-6/n-3 ratio	1.0 ± 0.0a	2.7 ± 0.0b	1.5 ± 0.0c	0.8 ± 0.0d	2.7 ± 0.0b	1.2 ± 0.0e
% n-6 HUFA	37 ± 0ad	64 ± 0b	43 ± 2a	31 ± 0cd	63 ± 0b	35 ± 1d

Hypothalamus –PL FA (ug/mg)	Medium fat (35en%)			High fat (60en%)
	1en%LA	8en% LA	8en% LA 1en% EPA+DHA	1en%LA	8en% LA	8en% LA 1en% EPA+DHA
18:2n-6	0.2 ±0.0	0.3 ±0.0	0.4 ± 0.1	0.2 ± 0.0	0.3 ± 0.0	0.4 ± 0.1
20:4n-6	3.3 ± 0.1ab	3.9 ± 0.1ac	3.7 ± 0.3abc	3.2 ± 0.1b	4.0 ±0.1c	3.6 ± 0.2abc
20:5n-3	0.04 ± 0.00abc	0.02 ± 0.00a	0.05 ± 0.01abc	0.05 ± 0.00bc	0.03 ± 0.01ac	0.06 ± 0.01c
22:5n-3	0.2 ± 0.0a	0.1 ± 0.0b	0.2 ± 0.0ac	0.2 ± 0.0c	0.1 ± 0.0b	0.2 ± 0.0c
22:6n-3	5.9 ± 0.2	6.0 ± 0.2	6.4 ± 0.5	6.0 ± 0.2	6.1 ± 0.2	6.3 ± 0.4
n-6/n-3 ratio	0.8 ± 0.0a	1.0 ± 0.0b	0.9 ±0.0a	0.8 ±0.0c	1.0 ±0.0b	0.9 ± 0.0a
n-6 HUFA (%)	43 ± 0ac	47 ± 0b	44 ± 1a	41 ±0c	48 ± 0b	43 ± 0a

% n-6 HUFA was calculated from the Lands equation [8] (20:3n-6 + 20:4n-6 + 22:5n-6)/(20:3n-6 + 20:4n-6 + 22:5n-6 + 20:5n-22:5n-3 + 22:6n-3)*100.

Different letter indicate significant statistical difference, Mann-Whitney p < 0.01, n = 8–10.

Data are presented as ± SEM. The full fatty acid profile of liver-PL and hypothalamus-PL is given in Supplementary Table 3.