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. Author manuscript; available in PMC: 2005 Nov 23.
Published in final edited form as: J Gen Physiol. 2005 Mar 14;125(4):377–394. doi: 10.1085/jgp.200409228

Figure 10.

Figure 10

The K464A mutation shortens the locked-open time of E1371S-CFTR. (A) Sample trace of K464A/E1371S-CFTR channels in the presence of 1 mM ATP + PKA. Note that the relaxation upon nucleotide washout is very fast. (B) Ensemble macroscopic currents were generated from 18 patches. The macroscopic current has a relaxation time constant of 19.60 ± 0.01 s (red curve). (C) Sample trace of K464A/E1371S-CFTR channels in the presence of 10 μM ATP (blue curve). The inset shows the presence of numerous brief openings (from 42 to 650 ms). Note also the presence of longer openings (*, 9 s).